A New Fluorescent Dye for Cell Tracing and Mitochondrial Imaging In Vitro and In Vivo

Press, Adrian; Ungelenk, Luisa; Rinkenauer, Alexandra; Gröger, Marko; Lehmann, Frank; Mosig, Alexander; Schubert, Ulrich S.; Clemens, Mark G.; Bauer, Michael

Abstract:

Mitochondria contribute to redox and calcium balance, and apoptosis thus regulating cellular fate. In the present study, mitochondrial staining applying a novel dye, V07-07059, was performed in human embryonic kidney cells, a human vascular endothelial cell line and primary human mononuclear cells. The new fluorescent mega Stokes dye (peak excitation: 488 nm, peak emission: 554 nm) showed superior fluorescent properties and stability. V07-07059 stains mitochondria dependent on their membrane potential and is safe to use in vitro and in vivo. Unlike other dyes applied in this context (e.g. Tetramethylrhodamine methyl ester), V07-07059 only marginally inhibits mitochondrial respiration and function. V07-07059 enables real time imaging of mitochondrial trafficking and remodeling. Prolonged staining with V07-07059 demonstrated the dyes suitability as a novel probe to track cells. In comparison to the widely used standard for cell proliferation and tracking studies 5(6)-diacetate N-succinimidyl ester, V07-07059 proved superior regarding toxicity and photostability.

Research areas:

• Biotechnology

Year:

2016

Type of Publication:

Article

Journal:

Journal of Biophotonics

Number:

9

Pages:

888 - 900

DOI:

10.1002/jbio.201500190