Automated MALDI-TOF-MS Sample Preparation in Combinatorial Polymer Research
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Meier, Michael; Hoogenboom, Richard; Fijten, Martin W. M.; Schneider, Michael; Schubert, Ulrich S.
- Abstract:
- A new automated matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDITOF-
MS) sample spotting technique that allows the integration of MALDI sample preparation in the workflow
of combinatorial polymer research is described. The technique is performed utilizing a commercially available
synthetic robot and was first evaluated with polymer standards of known composition and later on used for
the monitoring of the living cationic ring-opening polymerization of 2-ethyl-2-oxazoline. The spotting was
carried out as a multiple layer approach, which offers the ability of complex sample preparation without the
requirement of premixing the different components. The described technique reduces the time required for
sample preparation and offers the possibility of automated sample spotting during polymerization reactions
performed in a synthetic robot. This allows the integration of molecular weight screening and polymer
end/group determination utilizing MALDI-TOF-MS as a high-throughput tool in combinatorial polymer
research.
- Year:
- 2003
- Type of Publication:
- Article
- Journal:
- Journal of Combinatorial Chemistry
- Volume:
- 5
- Pages:
- 369 – 374