Statistical Approach To Understand MALDI-TOFMS Matrices: Discovery and Evaluation of New MALDI Matrices
-
Meier, Michael; Adams, Nico; Schubert, Ulrich S.
- Abstract:
- A statistical approach is described to better understand
the role of the matrix during a MALDI-TOFMS experiment.
Potential matrix molecules were selected based on
a rational experimental design and subsequently screened
in order to investigate whether a certain compound can
act as a matrix for synthetic polymers. The tested compounds
were selected from a pool of 11 000 molecules
based on descriptor calculations and a subsequent Doptimal
design selection procedure. This approach selected
59 organic compounds that were then investigated
for their ability to act a matrix for synthetic polymers in
MALDI-TOFMS experiments. Within this contribution, we
focus on the discussion of new MALDI-TOFMS matrixes
that were discovered in the course of the screening. At
least 10 new matrices were identified, and 5 of these were
subsequently tested more closely with a variety of different
synthetic polymers of different molecular weights revealing
good to excellent performance of these new matrices.
- Year:
- 2007
- Type of Publication:
- Article
- Journal:
- Analytical Chemistry
- Volume:
- 79
- Number:
- 3
- Pages:
- 863 - 869