Depicting the Spatial Distribution of Proteins in Human Tumor Tissue Combining SELDI, MALDI Imaging and Immunihistochemistry
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Wehder, Liane; G\", ; unther Ernst, ; Crecelius, Anna; Guntinas-Lichius, Orlando; Melle, Christian; Schubert, Ulrich S.; von Eggeling, Ferdinand
- Abstract:
- Carcinoma tissue consists of not only tumor cells but also fibroblasts, endothelial
cells or vascular structures, and inflammatory cells forming the supportive tumor stroma.
Therefore, the spatial distribution of proteins that promote growth and proliferation in
these complex functional units is of high interest. Matrix-assisted laser desorption/ionization
imaging mass spectrometry is a newly developed technique that generates spatially resolved
profiles of protein signals directly from thin tissue sections. Surface-enhanced laser desorption/
ionization mass spectrometry (MS)combined with tissue microdissection allows analysis of
defined parts of the tissue with a higher sensitivity and a broader mass range. Nevertheless,
both MS-based techniques have a limited spatial resolution. IHC is a technique that allows a
resolution down to the subcellular level. However, the detection and measurement of a specific
protein expression level is possible only by semiquantitative methods. Moreover, prior
knowledge about the identity of the proteins of interest is necessary. In this study, we combined
all three techniques to gain highest spatial resolution, sensitivity, and quantitative
information. We used frozen tissue from head and neck tumors and chose two exemplary
proteins (HNP1–3 and S100A8) to highlight the advantages and disadvantages of each technique.
It could be shown that the combination of these three techniques results in congruent
but also synergetic data.
- Year:
- 2010
- Type of Publication:
- Article
- Journal:
- Journal of Histochemisrty and Cytochemistry
- Volume:
- 58
- Pages:
- 929 - 937