Functionalized, Biocompatible Coating for Superparamagnetic Nanoparticles by Controlled Polymerization of a Thioglycosidic Monomer
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Babiuch, Krzysztof; Wyrwa, Ralf; Wagner, Kerstin; Seeman, Thomas; Höppener, Stephanie; Becer, C. Remzi; Linke, Ralf; Gottschaldt, Michael; J\", ; urgen Weisser, ; Schnabelrauch, Matthias; Schubert, Ulrich S.
- Abstract:
- It is demonstrated that water-soluble, glucosylated
poly(pentafluorostyrene) derivatives revealed favorable
coating material properties for magnetic iron oxide
nanoparticles. To prepare the coating material in high reproducibility
and purity as well as in sufficient amounts, a new
route of synthesis is established. The preparation and characterization
of the glucosylated, tetrafluorostyryl monomer,
by thiol-para-fluorine “click” reaction, and its polymerization,
via nitroxide-mediated radical process, is presented in detail.
In addition, the coating material and the resulting particle
properties are investigated by means of XPS, DLS, TGA, TEM, and cryo-TEM as well as flow cytometry. The glycopolymer acts as
an appropriate stabilizing agent for the superparamagnetic nanoparticles by the formation of an approximately 10 nm thick shell, as
shown by the XPS analysis. Furthermore, the application of FITC-labeled glycopolymer yielded fluorescent, superparamagnetic
nanoparticles, which can be used for monitoring cell-carbohydrate interactions, because these particles show no cytotoxicity
toward 3T3 fibroblasts.
- Year:
- 2011
- Type of Publication:
- Article
- Journal:
- Biomacromolecules
- Volume:
- 12
- Pages:
- 681 - 691