'One Cell-One Well' - A New Approach to Inkjet Printing Single Cell Microarrays
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Liberski, Albert R.; Delaney, Joseph T.; Schubert, Ulrich S.
- Abstract:
- A new approach to prepare arrays of sessile droplets
of living single cell cultures using a liquid hydrophobic barrier prevents
the samples from dehydrating, and allows for spatially addressable
arrays for statistical quantitative single cell studies. By carefully
advancing a thin layer ofmineral oil on the substrate over the droplets
during the printing, dehydration of the droplets can be prevented, and
the vitality of the cells can be maintained. The net result of this
confluence of submerged cell culturing and inkjet printing is facile
access to spatially addressable arrays of isolated single cells on surfaces.
Such single cell arrays may be particularly useful as high-throughput tools in the rapidly emerging “omics” fields of cell biology.
- Year:
- 2011
- Type of Publication:
- Article
- Keywords:
- one cell-one well; inkjet printing; single cell microarrays; microdroplets
- Journal:
- ACS Combinatorial Science
- Volume:
- 13
- Pages:
- 190 - 195