Combinatorial Optimization of Multiple MALDI Matrices on a Single Tissue Sample Using Inkjet Printing
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Delaney, Joseph T.; Urbanek, Annett; Wehder, Liane; Perelaer, Jolke; Crecelius, Anna; von Eggeling, Ferdinand; Schubert, Ulrich S.
- Abstract:
- Taking advantage of the drop-on-demand capabilities
of inkjet printing, the first example of a single tissue
being used as a substrate for preparing combinatorial arrays of
different matrix-assisted laser desorption/ionization (MALDI)
matrices in multiple concentrations on a single chip is reported.
By varying the number of droplets per spot that were printed, a
gradient array of different amounts of matrix material could be
printed on a single chip, while the selection of matrices could be adjusted by switching different matrix materials. The result was a
two-dimensional array of multiple matrices on a single tissue slice, which could be analyzed microscopically and by MALDI to
elucidate which combination of matrix and printing conditions offered the best resolution in terms of spot-to-spot distance and
signal-to-noise ratios for proteins in the recorded MS spectra. This combinatorial approach enables the efficient optimization of
possible matrices in an organized, side-by-side array format, which can particularly be useful for the detection of specific protein
markers.
- Year:
- 2011
- Type of Publication:
- Article
- Keywords:
- combinatorial chemistry; mass spectrometry; proteomics; surface analysis; MALDI-MSI; inkjet printing
- Journal:
- ACS Combinatorial Science
- Volume:
- 13
- Pages:
- 218 - 222