Fast High-throughput screening of temoporfin Loaded Liposomal Formulations Prepared by Ethanol injection Method
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Yang, Kewei; Delaney, Joseph T.; Schubert, Ulrich S.; Fahr, Alfred
- Abstract:
- A new strategy for fast, convenient high-throughput screening of liposomal formulations was developed, utilizing
the automation of the so-called ethanol-injection method. This strategy was illustrated by the preparation and
screening of the liposomal formulation library of a potent second-generation photosensitizer, temoporfin. Numerous
liposomal formulations were efficiently prepared using a pipetting robot, followed by automated size characterization,
using a dynamic light scattering plate reader. Incorporation efficiency of temoporfin and zeta potential were also
detected in selected cases. To optimize the formulation, different parameters were investigated, including lipid
types, lipid concentration in injected ethanol, ratio of ethanol to aqueous solution, ratio of drug to lipid, and the
addition of functional phospholipid. Step-by-step small liposomes were prepared with high incorporation efficiency.
At last, an optimized formulation was obtained for each lipid in the following condition: 36.4 mg·mL−1 lipid, 13.1
mg·mL−1 mPEG2000-DSPE, and 1:4 ethanol:buffer ratio. These liposomes were unilamellar spheres, with a diameter of
approximately 50 nm, and were very stable for over 20 weeks. The results illustrate this approach to be promising for
fast high-throughput screening of liposomal formulations.
- Year:
- 2012
- Type of Publication:
- Article
- Keywords:
- Liposome; ethanol injection method; temoporfin; ethosome; pipetting robot
- Journal:
- Journal of Lipid Research
- Volume:
- 22
- Pages:
- 31 - 41