Preparation, Cellular Internalization and Biocompatibility of Highly Fluorescent PMMA Nanoparticles
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Vollrath, Antje; Pretzel, David; Pietsch, Christian; Perevyazko, Igor; Schubert, Stephanie; Pavlov, George M.; Schubert, Ulrich S.
- Abstract:
- Methacrylate monomers were functionalized with a 4-hydroxythiazole chromophore and copolymerized
with methyl methacrylate via RAFT. Nanoparticles of 120 and 500 nm in size were
prepared without using stabilizers/surfactants. For comparative studies, preparative ultracentrifugation
was applied for the separation into small and large
particle fractions. All suspensions were characterized by DLS,
AUC, and SEM and tested regarding their stability during centrifugation
and re-suspension, autoclavation, and incubation
in cell culture media. In vitro studies with mouse fi broblast
cell line and differently sized NP showed a particle uptake into
cells. Biocompatibility, non-toxicity, and hemocompatibility
were demonstrated using a XTT assay, a live/dead staining,
and an erythrocyte aggregation and hemolysis assay.
- Year:
- 2012
- Type of Publication:
- Article
- Journal:
- Macromolecular Rapid Communications
- Volume:
- 33
- Pages:
- 1791 - 1797